Bio and Environmental Technology HOMEResearchBiotechnology
Bio and Environmental Technology
The integration of chemical engineering principles with biology has seen synergistic effects on better understanding and analysis of exquisite biochemical phenomena, facilitating their engineering application in virtually all areas.

Our research is directed towards the scalable protein refolding process design, biomineralization bsed hybrid nanoparticle synthesis, bioprocess intensification, biosensors and bioelectronics, design and application of engineered biomolecules, bioinspired nanopatterning, bioremediation, bioenergy, bioremediation and bionano analytical device design.

Many ongoing projects are of multidisciplinary nature, emphasizing active collaboration among the researchers of diverse backgrounds.
단백질 및 생물공정공
신생체모사재료 및 면역
나노생체재료 재생의학
세포 및 조직공학
Biomolecualr & Bioprocess Engineering
Major Research Areas
Refolding of IB proteins using folding-like-refolding strategy
Refolding is a key issue to determine the overall success of therapeutic protein production via IB route using E. coli. Regardless of target protein property and process scale, protein concentration at the time of refolding is a crucial factor to determine refolding efficiency. In this context, practical engineering approach to improve refolding efficiency would be to create an environment to control protein interaction in a defined manner. Deposition of denatured protein onto a surface is expected to minimize mutual interactions of proteins. Surface refolding can be easily integrated with the novel in-house IB processing scheme without increasing the process complexity while achieving significant process intensification. Furthermore, we recently developed a novel refolding strategy (i.e. folding-like-refolding) harnessing refolding cocktail comprising unpurified chaperones to effectively mimic in vivo protein quality control network. It is expected that this new strategy will provide an economical viable refolding method for a wide variety of refolding recalcitrant proteins.

Design of nanostructured materials via protein engineering
This research aims to design various nanostructured materials guided by a nanoscale novel protein/DNA scaffold as an alternative to existing physicochemical approaches. Phage display or SELEX technique has found increasing applications in the screening of random peptide/aptamer sequences exhibiting specific affinity towards many interesting functional inorganic materials. Identified aptamers of desired characteristics are fused to generate novel proteins without affecting the structure or function of the original protein. The engineered proteins are expected to facilitate the nanopatterning of functional inorganics in a controlled manner. The peptide or apramer also can find interesting application in affinity ligand design or biomineralization of hybrid functional materials.

Intensification of plasmid DNA Vaccine Purification
Separation of negatively charged molecules, such as plasmid DNA (pDNA), RNA and endotoxin forms a bottleneck for the development of pDNA vaccine production process. The use of affinity interactions of transition metal ions with these molecules may provide an ideal separation methodology. We have been studying differential binding behaviour of pDNA, RNA and endotoxin to transition metal ions. Interestingly, the interactions of immobilized and/or free metal ions with pDNA, RNA and endotoxin showed a hierarchical binding pattern, to develop a simplified pDNA purification process with improved process economics.

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